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- ************************************************************************
- * Binding-protein-dependent transport systems inner membrane component *
- * signature *
- ************************************************************************
-
- Bacterial binding protein-dependent transport systems [1,2] are multicomponent
- systems typically composed of a periplasmic substrate-binding protein, one or
- two reciprocally homologous integral inner-membrane proteins and one or two
- peripheral membrane ATP-binding proteins that couple energy to the active
- transport system.
-
- The integral inner-membrane proteins translocate the substrate across the
- membrane. It has been shown [3] that most of these proteins contain a
- conserved region located about 80 to 100 residues from their C-terminal
- extremity. This region seems [4] to be located in a cytoplasmic loop between
- two transmembrane domains. Proteins belonging to this family are listed
- below (references are only provided for recently sequenced proteins).
-
- Name(s) Transport system
- ----------- --------------------------------------
- amiC amiD Oligopeptides
- artM artQ Arginine
- cysT cysW Sulfate / thiosulfate
- dciAB dciAC Dipeptides
- hisM hisQ Histidine
- malF malG Maltose
- msmF msmG Melibiose / raffinose / isomaltotriose
- modB Molybdenum
- nifC Molybdenum (?)
- nikB nikC Nickel
- oppB oppC Oligopeptides
- phnM Alkylphosphonate (?)
- potB potC Spermidine/putrescine
- potH potI Putrescine [5]
- proW Glycine betaine / L-proline
- pstA pstC Phosphate
- sapB sapC Peptides [6]
- ugpA ugpE sn-glycerol-3-phosphate
-
- mbpY Unknown, from chloroplast of Marchantia polymorpha
- yehW yehY Unknown, from Escherichia coli
-
- We built a signature pattern from the best conserved positions within the
- conserved region.
-
- -Consensus pattern: [LIVMFY]-x(8)-[EQR]-[STAV]-[STAG]-x(3)-G-[LIVMFYSTAC]-
- x(5)-[LIVMFYSTA]-x(4)-[LIVMFY]-[PKR]
- -Sequences known to belong to this class detected by the pattern: the majority
- of these proteins with the exception of amiC, amiD, artM, dciAC, lacG, nikB,
- nikC, phnM, sapB, and sapC.
- -Other sequence(s) detected in SWISS-PROT: 26.
- -Last update: June 1994 / Pattern and text revised.
-
- [ 1] Ames G.F.-L.
- Annu. Rev. Biochem. 55:397-425(1986).
- [ 2] Higgins C.F., Hyde S.C., Mimmack M.M., Gileadi U., Gill D.R.,
- Gallagher M.P.
- J. Bioenerg. Biomembr. 22:571-592(1990).
- [ 3] Dassa E., Hofnung M.
- EMBO J. 4:2287-2293(1985).
- [ 4] Pearce S.R., Mimmack M.L., Gallagher M.P., Gileadi U., Hyde S.C.,
- Higgins C.F.
- Mol. Microbiol. 6:57-57(1992).
- [ 5] Pistocchi R., Kashiwagi K., Miyamoto S., Nukui E., Sadakata Y.,
- Kobayashi H., Igarashi K.
- J. Biol. Chem. 268:146-152(1993).
- [ 6] Parra-Lopez C., Baer M.T., Groisman E.A.
- EMBO J. 12:4053-4062(1993).
-